An enhanced chimeric firefly luciferase-inspired enzyme for ATP detection and bioluminescence reporter and imaging applications

Firefly luciferases, which emit visible light in a highly specific ATP-dependent process, have been adapted for a variety of applications including gene reporter assays, whole-cell biosensor measurements and in vivo imaging. We have previously reported the ~2-fold enhanced activity and 1.4-greater b...

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Bibliografische gegevens
Gepubliceerd in:Anal Biochem
Hoofdauteurs: Branchini, Bruce R., Southworth, Tara L., Fontaine, Danielle M., Kohrt, Dawn, Talukder, Munya, Michelini, Elisa, Cevenini, Luca, Roda, Aldo, Grossel, Martha J.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2015
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4496250/
https://ncbi.nlm.nih.gov/pubmed/26049097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.05.020
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