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Continuous directed evolution of aminoacyl-tRNA synthetases

Directed evolution of orthogonal aminoacyl-tRNA synthetases (AARSs) enables site-specific installation of non-canonical amino acids (ncAAs) into proteins. Traditional evolution techniques typically produce AARSs with greatly reduced activity and selectivity compared to their wild-type counterparts....

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Publicat a:Nat Chem Biol
Autors principals: Bryson, David I., Fan, Chenguang, Guo, Li-Tao, Miller, Corwin, Söll, Dieter, Liu, David R.
Format: Artigo
Idioma:Inglês
Publicat: 2017
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5724969/
https://ncbi.nlm.nih.gov/pubmed/29035361
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2474
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