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Continuous directed evolution of aminoacyl-tRNA synthetases
Directed evolution of orthogonal aminoacyl-tRNA synthetases (AARSs) enables site-specific installation of non-canonical amino acids (ncAAs) into proteins. Traditional evolution techniques typically produce AARSs with greatly reduced activity and selectivity compared to their wild-type counterparts....
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| Опубликовано в: : | Nat Chem Biol |
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| Главные авторы: | , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2017
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5724969/ https://ncbi.nlm.nih.gov/pubmed/29035361 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2474 |
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