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Generation and CRISPR/Cas9 editing of transformed progenitor B cells as a pseudo-physiological system to study DNA repair gene function in V(D)J recombination

Antigen receptor gene assembly is accomplished in developing lymphocytes by the V(D)J recombination reaction, which can be separated into two steps: DNA cleavage by the recombination-activating gene (RAG) nuclease and joining of DNA double strand breaks (DSBs) by components of the nonhomologous end...

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Dettagli Bibliografici
Pubblicato in:J Immunol Methods
Autori principali: Lenden Hasse, Hélène, Lescale, Chloé, Bianchi, Joy J., Yu, Wei, Bedora-Faure, Marie, Deriano, Ludovic
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5714433/
https://ncbi.nlm.nih.gov/pubmed/28882611
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2017.08.007
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