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Thiol-linked alkylation of RNA to assess expression dynamics
Gene expression profiling by high-throughput sequencing reveals qualitative and quantitative changes in RNA species at steady-state but obscures the intracellular dynamics of RNA transcription, processing and decay. We developed thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM-s...
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| Udgivet i: | Nat Methods |
|---|---|
| Main Authors: | , , , , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5712218/ https://ncbi.nlm.nih.gov/pubmed/28945705 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4435 |
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