Sequencing cell type-specific transcriptomes with SLAM-ITseq

Analysis of cell type-specific transcriptomes is vital for understanding the biology of tissues and organs in the context of multicellular organisms. Here, we describe a protocol to metabolically label RNA with a uracil analogue, 4-thiouracil, in specific cell types in vivo, and a pipeline to detect...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nat Protoc
Prif Awduron: Matsushima, Wayo, Herzog, Veronika A, Neumann, Tobias, Gapp, Katharina, Zuber, Johannes, Ameres, Stefan L, Miska, Eric A
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2019
Pynciau:
Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC6997029/
https://ncbi.nlm.nih.gov/pubmed/31243395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-019-0179-x
Tagiau: Ychwanegu Tag
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!

Eitemau Tebyg