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Sequencing cell type-specific transcriptomes with SLAM-ITseq
Analysis of cell type-specific transcriptomes is vital for understanding the biology of tissues and organs in the context of multicellular organisms. Here, we describe a protocol to metabolically label RNA with a uracil analogue, 4-thiouracil, in specific cell types in vivo, and a pipeline to detect...
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| Publicado no: | Nat Protoc |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6997029/ https://ncbi.nlm.nih.gov/pubmed/31243395 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-019-0179-x |
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