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Mutation of nucleotides around the +1 position of type 3 polymerase III promoters: The effect on transcriptional activity and start site usage

Type 3 RNA polymerase III (Pol III) promoters are widely used for the expression of small RNAs such as short hairpin RNA and guide RNA in the popular RNAi and CRISPR-Cas gene regulation systems. Although it is generally believed that type 3 Pol III promoters use a defined transcription start site (+...

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Publicat a:Transcription
Autors principals: Gao, Zongliang, Harwig, Alex, Berkhout, Ben, Herrera-Carrillo, Elena
Format: Artigo
Idioma:Inglês
Publicat: Taylor & Francis 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5703244/
https://ncbi.nlm.nih.gov/pubmed/28598252
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/21541264.2017.1322170
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