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Fluorophore Labeling, Nanodisc Reconstitution and Single-molecule Observation of a G Protein-coupled Receptor

Activation of G protein-coupled receptors (GPCRs) by agonist ligands is mediated by a transition from an inactive to active receptor conformation. We describe a novel single-molecule assay that monitors activation-linked conformational transitions in individual GPCR molecules in real-time. The recep...

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Bibliografski detalji
Izdano u:Bio Protoc
Glavni autori: Lamichhane, Rajan, Liu, Jeffrey J., Pauszek, Raymond F., Millar, David P.
Format: Artigo
Jezik:Inglês
Izdano: Bio-Protocol 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5697779/
https://ncbi.nlm.nih.gov/pubmed/29170748
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2332
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