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Sequence-specific fluorescent labeling of double-stranded DNA observed at the single molecule level
Fluorescent labeling of a short sequence of double-stranded DNA (dsDNA) was achieved by ligating a labeled dsDNA fragment to a stem–loop triplex forming oligonucleotide (TFO). After the TFO has wound around the target sequence by ligand-induced triple helix formation, its extremities hybridize to ea...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC219493/ https://ncbi.nlm.nih.gov/pubmed/14530458 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng125 |
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