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Sequence-specific fluorescent labeling of double-stranded DNA observed at the single molecule level

Fluorescent labeling of a short sequence of double-stranded DNA (dsDNA) was achieved by ligating a labeled dsDNA fragment to a stem–loop triplex forming oligonucleotide (TFO). After the TFO has wound around the target sequence by ligand-induced triple helix formation, its extremities hybridize to ea...

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書目詳細資料
Main Authors: Géron-Landre, Bénédicte, Roulon, Thibaut, Desbiolles, Pierre, Escudé, Christophe
格式: Artigo
語言:Inglês
出版: Oxford University Press 2003
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC219493/
https://ncbi.nlm.nih.gov/pubmed/14530458
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng125
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