Sequence-specific fluorescent labeling of double-stranded DNA observed at the single molecule level

Fluorescent labeling of a short sequence of double-stranded DNA (dsDNA) was achieved by ligating a labeled dsDNA fragment to a stem–loop triplex forming oligonucleotide (TFO). After the TFO has wound around the target sequence by ligand-induced triple helix formation, its extremities hybridize to ea...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Géron-Landre, Bénédicte, Roulon, Thibaut, Desbiolles, Pierre, Escudé, Christophe
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2003
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC219493/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14530458/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng125
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