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Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides
We report that engineered Cas9 variants with improved specificity—eCas9-1.1 and Cas9-HF1—are often poorly active in human cells, when complexed with single guide RNAs (sgRNAs) with a mismatch at the 5’ terminus, relative to target DNA sequences. Because the nucleotide at the 5’ end of sgRNAs, expres...
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| Publié dans: | Genome Biol |
|---|---|
| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
BioMed Central
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5686910/ https://ncbi.nlm.nih.gov/pubmed/29141659 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-017-1355-3 |
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