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CRISPR-Cas9(D10A) Nickase-Assisted Genome Editing in Lactobacillus casei
Lactobacillus casei has drawn increasing attention as a health-promoting probiotic, while effective genetic manipulation tools are often not available, e.g., the single-gene knockout in L. casei still depends on the classic homologous recombination-dependent double-crossover strategy, which is quite...
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| Publicado no: | Appl Environ Microbiol |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5666132/ https://ncbi.nlm.nih.gov/pubmed/28864652 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01259-17 |
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