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CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri
Clustered regularly interspaced palindromic repeats (CRISPRs) and the CRISPR-associated (Cas) nuclease protect bacteria and archeae from foreign DNA by site-specific cleavage of incoming DNA. Type-II CRISPR–Cas systems, such as the Streptococcus pyogenes CRISPR–Cas9 system, can be adapted such that...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
2014
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4176153/ https://ncbi.nlm.nih.gov/pubmed/25074379 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku623 |
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