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CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

Clustered regularly interspaced palindromic repeats (CRISPRs) and the CRISPR-associated (Cas) nuclease protect bacteria and archeae from foreign DNA by site-specific cleavage of incoming DNA. Type-II CRISPR–Cas systems, such as the Streptococcus pyogenes CRISPR–Cas9 system, can be adapted such that...

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Main Authors: Oh, Jee-Hwan, van Pijkeren, Jan-Peter
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2014
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4176153/
https://ncbi.nlm.nih.gov/pubmed/25074379
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku623
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