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CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing
The RNA-guided Cas9 nuclease is being widely employed to engineer the genomes of various cells and organisms. Despite the efficient mutagenesis induced by Cas9, off-target effects have raised concerns over the system’s specificity. Recently a “double-nicking” strategy using catalytic mutant Cas9(D10...
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| Veröffentlicht in: | Sci Rep |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4832145/ https://ncbi.nlm.nih.gov/pubmed/27079678 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep24356 |
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