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Establishment of a novel hepatocyte model that expresses four cytochrome P450 genes stably via mammalian-derived artificial chromosome for pharmacokinetics and toxicity studies

The utility of HepG2 cells to assess drug metabolism and toxicity induced by chemical compounds is hampered by their low cytochrome P450 (CYP) activities. To overcome this limitation, we established HepG2 cell lines expressing major CYP enzymes involved in drug metabolism (CYP2C9, CYP2C19, CYP2D6, a...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Satoh, Daisuke, Iwado, Satoru, Abe, Satoshi, Kazuki, Kanako, Wakuri, Shinobu, Oshimura, Mitsuo, Kazuki, Yasuhiro
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5655360/
https://ncbi.nlm.nih.gov/pubmed/29065189
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0187072
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