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A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

Over the years, the yeast one-hybrid assay has proven to be an important technique for the identification and validation of physical interactions between proteins such as transcription factors (TFs) and their DNA target. The method presented here utilizes the underlying concept of the Y1H but is mod...

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書目詳細資料
發表在:J Vis Exp
Main Authors: Tripathi, Prateek, Pruneda-Paz, José L., Kay, Steve A.
格式: Artigo
語言:Inglês
出版: MyJove Corporation 2017
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5612592/
https://ncbi.nlm.nih.gov/pubmed/28784944
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56080
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