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Design of a single plasmid-based modified yeast one-hybrid system for investigation of in vivo protein-protein and protein-DNA interactions
We have developed a modified yeast one-hybrid system (MY1H) useful for in vivo investigation of protein-protein and protein-DNA interactions. Our single-plasmid expression system is capable of differential protein expression levels; in addition to a GAL4 activation domain (AD) fusion protein, a seco...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2646854/ https://ncbi.nlm.nih.gov/pubmed/18778253 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000112901 |
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