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A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

Over the years, the yeast one-hybrid assay has proven to be an important technique for the identification and validation of physical interactions between proteins such as transcription factors (TFs) and their DNA target. The method presented here utilizes the underlying concept of the Y1H but is mod...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Tripathi, Prateek, Pruneda-Paz, José L., Kay, Steve A.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5612592/
https://ncbi.nlm.nih.gov/pubmed/28784944
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56080
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