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A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies
Over the years, the yeast one-hybrid assay has proven to be an important technique for the identification and validation of physical interactions between proteins such as transcription factors (TFs) and their DNA target. The method presented here utilizes the underlying concept of the Y1H but is mod...
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| Published in: | J Vis Exp |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
MyJove Corporation
2017
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5612592/ https://ncbi.nlm.nih.gov/pubmed/28784944 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56080 |
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