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A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

Over the years, the yeast one-hybrid assay has proven to be an important technique for the identification and validation of physical interactions between proteins such as transcription factors (TFs) and their DNA target. The method presented here utilizes the underlying concept of the Y1H but is mod...

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Foilsithe in:J Vis Exp
Main Authors: Tripathi, Prateek, Pruneda-Paz, José L., Kay, Steve A.
Formáid: Artigo
Teanga:Inglês
Foilsithe: MyJove Corporation 2017
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5612592/
https://ncbi.nlm.nih.gov/pubmed/28784944
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56080
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