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Novel cell surface luciferase reporter for high-throughput yeast one-hybrid screens

Gene-centered yeast one-hybrid (Y1H) screens provide a powerful and effective strategy to identify transcription factor (TF)-promoter interactions. While genome-wide TF ORFeome clone collections are increasingly available, screening protocols have limitations inherent to the properties of the enzyma...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Bonaldi, Katia, Li, Zheng, Kang, S. Earl, Breton, Ghislain, Pruneda-Paz, Jose L.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5737895/
https://ncbi.nlm.nih.gov/pubmed/28985361
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx682
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