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A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris
Pichia pastoris is widely used as a platform for heterologous protein expression because of its high volumetric productivity. Multicopy integration of the target gene is commonly used to improve the production of the target protein. Cre/lox recombination system is a powerful tool for the marker resc...
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| Publicado no: | Front Microbiol |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Frontiers Media S.A.
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5601908/ https://ncbi.nlm.nih.gov/pubmed/28955309 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.01698 |
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