Increase of Candida antarctica lipase B production under PGK promoter in Pichia pastoris: effect of multicopies

The effect of gene dosage on the production of Candida antarctica lipase B (CalB) in the methylotrophic yeast Komagataella phaffii, at high densities in a simple medium containing crude glycerin as the sole carbon source, is described. The use of crude glycerin, the main by-product of biodiesel prod...

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Detalhes bibliográficos
Publicado no:Braz J Microbiol
Main Authors: Robert, Julia Macedo, Betancur, Maritza Ocampo, Machado, Antonio Carlos Oliveira, Arruda, Andrelisse, Reis, Viviane Castelo Branco, Almeida, Rodrigo Volcan, Torres, Fernando Araripe Gonçalves, Alegre, Pau Ferrer, Valero, Francisco, Freire, Denise Maria Guimarães
Formato: Artigo
Idioma:Inglês
Publicado em: Springer International Publishing 2019
Assuntos:
Biotechnology and Industrial Microbiology - Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6863280/
https://ncbi.nlm.nih.gov/pubmed/30827000
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s42770-019-00056-8
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