Increase of Candida antarctica lipase B production under PGK promoter in Pichia pastoris: effect of multicopies

The effect of gene dosage on the production of Candida antarctica lipase B (CalB) in the methylotrophic yeast Komagataella phaffii, at high densities in a simple medium containing crude glycerin as the sole carbon source, is described. The use of crude glycerin, the main by-product of biodiesel prod...

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Dades bibliogràfiques
Publicat a:Braz J Microbiol
Autors principals: Robert, Julia Macedo, Betancur, Maritza Ocampo, Machado, Antonio Carlos Oliveira, Arruda, Andrelisse, Reis, Viviane Castelo Branco, Almeida, Rodrigo Volcan, Torres, Fernando Araripe Gonçalves, Alegre, Pau Ferrer, Valero, Francisco, Freire, Denise Maria Guimarães
Format: Artigo
Idioma:Inglês
Publicat: Springer International Publishing 2019
Matèries:
Biotechnology and Industrial Microbiology - Research Paper
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6863280/
https://ncbi.nlm.nih.gov/pubmed/30827000
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s42770-019-00056-8
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