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A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris

Pichia pastoris is widely used as a platform for heterologous protein expression because of its high volumetric productivity. Multicopy integration of the target gene is commonly used to improve the production of the target protein. Cre/lox recombination system is a powerful tool for the marker resc...

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Dettagli Bibliografici
Pubblicato in:Front Microbiol
Autori principali: Li, Ding, Zhang, Bo, Li, Shuting, Zhou, Jie, Cao, Hui, Huang, Yan, Cui, Zhongli
Natura: Artigo
Lingua:Inglês
Pubblicazione: Frontiers Media S.A. 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5601908/
https://ncbi.nlm.nih.gov/pubmed/28955309
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.01698
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