A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris

Pichia pastoris is widely used as a platform for heterologous protein expression because of its high volumetric productivity. Multicopy integration of the target gene is commonly used to improve the production of the target protein. Cre/lox recombination system is a powerful tool for the marker resc...

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Detalles Bibliográficos
Publicado en:Front Microbiol
Autores principales: Li, Ding, Zhang, Bo, Li, Shuting, Zhou, Jie, Cao, Hui, Huang, Yan, Cui, Zhongli
Formato: Artigo
Lenguaje:Inglês
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5601908/
https://ncbi.nlm.nih.gov/pubmed/28955309
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.01698
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