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Engineering human PrimPol into an efficient RNA-dependent-DNA primase/polymerase
We have developed a straightforward fluorometric assay to measure primase-polymerase activity of human PrimPol (HsPrimPol). The sensitivity of this procedure uncovered a novel RNA-dependent DNA priming-polymerization activity (RdDP) of this enzyme. In an attempt to enhance HsPrimPol RdDP activity, w...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5587808/ https://ncbi.nlm.nih.gov/pubmed/28911121 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx633 |
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