Adenovirus-mediated Cre deletion of floxed sequences in primary mouse cells is an efficient alternative for studies of gene deletion

This study evaluates the utility of Cre-expressing adenovirus for deletion of floxed genes in primary cells using primary murine hepatocytes. Adenovirus infection was very efficient, even at very low MOI (>95% infection at a MOI of 6) and did not reduce viability. High level LacZ expression was cyto...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Prost, Sandrine, Sheahan, Sharon, Rannie, Dominic, Harrison, David J.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2001
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55864/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11504888/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.16.e80
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