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Adenovirus-mediated Cre deletion of floxed sequences in primary mouse cells is an efficient alternative for studies of gene deletion

This study evaluates the utility of Cre-expressing adenovirus for deletion of floxed genes in primary cells using primary murine hepatocytes. Adenovirus infection was very efficient, even at very low MOI (>95% infection at a MOI of 6) and did not reduce viability. High level LacZ expression was c...

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Bibliografische gegevens
Hoofdauteurs: Prost, Sandrine, Sheahan, Sharon, Rannie, Dominic, Harrison, David J.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2001
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC55864/
https://ncbi.nlm.nih.gov/pubmed/11504888
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