Adenovirus-mediated Cre deletion of floxed sequences in primary mouse cells is an efficient alternative for studies of gene deletion
This study evaluates the utility of Cre-expressing adenovirus for deletion of floxed genes in primary cells using primary murine hepatocytes. Adenovirus infection was very efficient, even at very low MOI (>95% infection at a MOI of 6) and did not reduce viability. High level LacZ expression was cyto...
محفوظ في:
| الحاوية / القاعدة: | Nucleic Acids Res |
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| المؤلفون الرئيسيون: | , , , |
| التنسيق: | Artigo |
| اللغة: | Inglês |
| منشور في: |
Oxford University Press
2001
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55864/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11504888/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.16.e80 |
| الوسوم: |
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