Reconstitution of beta-adrenergic receptor with components of adenylate cyclase.

Beta 1-Adrenergic receptor proteins were extracted from turkey erythrocyte membranes with lauroyl sucrose and digitonin and purified by affinity chromatography on a column of alprenolol agarose Affi-gel 10 or 15. The 5000-fold purified receptor is able to couple functionally with the stimulatory GTP...

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Detalles Bibliográficos
Publicado en:EMBO J
Main Authors: Hekman, M, Feder, D, Keenan, A K, Gal, A, Klein, H W, Pfeuffer, T, Levitzki, A, Helmreich, E J
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group 1984
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC557860/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6098472/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1984.tb02301.x
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