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Optical microscope illumination analysis using through-focus scanning optical microscopy
Misalignment of the aperture diaphragm present in optical microscopes results in angular illumination asymmetry (ANILAS) at the sample plane. Here we show that through-focus propagation of ANILAS results in lateral image shift with focus position. This could lead to substantial errors in quantitativ...
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| Publicado no: | Opt Lett |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5572797/ https://ncbi.nlm.nih.gov/pubmed/28614338 |
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