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Improved tandem affinity purification tag and methods for isolation of proteins and protein complexes from S. pombe
The Tandem Affinity Purification (TAP) method uses an epitope that contains two different affinity purification tags separated by a site-specific protease site to isolate a protein rapidly and easily. Proteins purified via the TAP tag are eluted under mild conditions, allowing them to be used for st...
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| Publicado no: | Cold Spring Harb Protoc |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5570513/ https://ncbi.nlm.nih.gov/pubmed/28250214 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot091611 |
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