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Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method
Affinity purification approaches have been successful in isolating native complexes for proteomic characterization. Structural heterogeneity and a degree of compositional heterogeneity of a complex do not usually impede progress in conducting such studies. In contrast, a complex intended for structu...
Bewaard in:
| Gepubliceerd in: | J Vis Exp |
|---|---|
| Hoofdauteurs: | , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
MyJove Corporation
2016
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5091696/ https://ncbi.nlm.nih.gov/pubmed/27501074 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54389 |
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