An improved strategy for tandem affinity purification-tagging of Schizosaccharomyces pombe genes

Tandem affinity purification (TAP) is a method that allows rapid purification of native protein complexes. We developed an improved technique to fuse the fission yeast genes with a TAP tag. Our technique is based on tagging constructs that contain regions homologous to the target gene cloned into ve...

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Библиографические подробности
Главные авторы: Cipak, Lubos, Spirek, Mario, Novatchkova, Maria, Chen, Zhiming, Rumpf, Cornelia, Lugmayr, Wolfgang, Mechtler, Karl, Ammerer, Gustav, Csaszar, Edina, Gregan, Juraj
Формат: Artigo
Язык:Inglês
Опубликовано: 2009
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Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2956173/
https://ncbi.nlm.nih.gov/pubmed/19750511
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.200800948
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