טוען...
An improved strategy for tandem affinity purification-tagging of Schizosaccharomyces pombe genes
Tandem affinity purification (TAP) is a method that allows rapid purification of native protein complexes. We developed an improved technique to fuse the fission yeast genes with a TAP tag. Our technique is based on tagging constructs that contain regions homologous to the target gene cloned into ve...
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| Main Authors: | , , , , , , , , , |
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| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
2009
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2956173/ https://ncbi.nlm.nih.gov/pubmed/19750511 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.200800948 |
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