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Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe
Reversible protein phosphorylation is a major regulatory mechanism in a cell. A chemical-genetic strategy to conditionally inactivate protein kinases has been developed recently. Mutating a single residue in the ATP-binding pocket confers sensitivity to small-molecule inhibitors. The inhibitor can o...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2957860/ https://ncbi.nlm.nih.gov/pubmed/18007635 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2007.447 |
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