Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe

Reversible protein phosphorylation is a major regulatory mechanism in a cell. A chemical-genetic strategy to conditionally inactivate protein kinases has been developed recently. Mutating a single residue in the ATP-binding pocket confers sensitivity to small-molecule inhibitors. The inhibitor can o...

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Main Authors: Gregan, Juraj, Zhang, Chao, Rumpf, Cornelia, Cipak, Lubos, Li, Zhang, Uluocak, Pelin, Nasmyth, Kim, Shokat, Kevan M
Format: Artigo
Jezik:Inglês
Izdano: 2007
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2957860/
https://ncbi.nlm.nih.gov/pubmed/18007635
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2007.447
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