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Tandem affinity purification of functional TAP-tagged proteins from human cells
Tandem affinity purification (TAP) is a generic two-step affinity purification protocol for isolation of TAP-tagged proteins together with associated proteins. We used bacterial artificial chromosome to heterologously express TAP-tagged murine Sgo1 protein in human HeLa cells. This allowed us to tes...
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| 主要な著者: | , , , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2007
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2957861/ https://ncbi.nlm.nih.gov/pubmed/17546005 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2007.172 |
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