Tandem affinity purification of functional TAP-tagged proteins from human cells

Tandem affinity purification (TAP) is a generic two-step affinity purification protocol for isolation of TAP-tagged proteins together with associated proteins. We used bacterial artificial chromosome to heterologously express TAP-tagged murine Sgo1 protein in human HeLa cells. This allowed us to tes...

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Main Authors: Gregan, Juraj, Riedel, Christian G, Petronczki, Mark, Cipak, Lubos, Rumpf, Cornelia, Poser, Ina, Buchholz, Frank, Mechtler, Karl, Nasmyth, Kim
Formato: Artigo
Idioma:Inglês
Publicado: 2007
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2957861/
https://ncbi.nlm.nih.gov/pubmed/17546005
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2007.172
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