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Tandem affinity purification of functional TAP-tagged proteins from human cells
Tandem affinity purification (TAP) is a generic two-step affinity purification protocol for isolation of TAP-tagged proteins together with associated proteins. We used bacterial artificial chromosome to heterologously express TAP-tagged murine Sgo1 protein in human HeLa cells. This allowed us to tes...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2957861/ https://ncbi.nlm.nih.gov/pubmed/17546005 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2007.172 |
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