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Affinity Purification of Protein Complexes Using TAP Tags
This protocol is used for the isolation and analysis of protein complexes using the tandem affinity purification (TAP) tag system. The protocol describes the purification of a protein fused to a TAP tag comprised of two protein A domains and the calmodulin binding peptide separated by a TEV cleavage...
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| Izdano u: | Methods Enzymol |
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| Glavni autori: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2015
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4794980/ https://ncbi.nlm.nih.gov/pubmed/26096502 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2014.11.007 |
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