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Affinity Purification of Protein Complexes Using TAP Tags

This protocol is used for the isolation and analysis of protein complexes using the tandem affinity purification (TAP) tag system. The protocol describes the purification of a protein fused to a TAP tag comprised of two protein A domains and the calmodulin binding peptide separated by a TEV cleavage...

詳細記述

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書誌詳細
出版年:Methods Enzymol
主要な著者: Gerace, Erica, Moazed, Danesh
フォーマット: Artigo
言語:Inglês
出版事項: 2015
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC4794980/
https://ncbi.nlm.nih.gov/pubmed/26096502
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2014.11.007
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