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Affinity Purification of Protein Complexes Using TAP Tags
This protocol is used for the isolation and analysis of protein complexes using the tandem affinity purification (TAP) tag system. The protocol describes the purification of a protein fused to a TAP tag comprised of two protein A domains and the calmodulin binding peptide separated by a TEV cleavage...
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| 出版年: | Methods Enzymol |
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| 主要な著者: | , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2015
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4794980/ https://ncbi.nlm.nih.gov/pubmed/26096502 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2014.11.007 |
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