Loading...

Affinity Purification of Protein Complexes Using TAP Tags

This protocol is used for the isolation and analysis of protein complexes using the tandem affinity purification (TAP) tag system. The protocol describes the purification of a protein fused to a TAP tag comprised of two protein A domains and the calmodulin binding peptide separated by a TEV cleavage...

Full description

Saved in:
Bibliographic Details
Published in:Methods Enzymol
Main Authors: Gerace, Erica, Moazed, Danesh
Format: Artigo
Language:Inglês
Published: 2015
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4794980/
https://ncbi.nlm.nih.gov/pubmed/26096502
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2014.11.007
Tags: Add Tag
No Tags, Be the first to tag this record!