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Affinity Purification of Protein Complexes Using TAP Tags

This protocol is used for the isolation and analysis of protein complexes using the tandem affinity purification (TAP) tag system. The protocol describes the purification of a protein fused to a TAP tag comprised of two protein A domains and the calmodulin binding peptide separated by a TEV cleavage...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Methods Enzymol
Main Authors: Gerace, Erica, Moazed, Danesh
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4794980/
https://ncbi.nlm.nih.gov/pubmed/26096502
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2014.11.007
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