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Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads
FLAG is an affinity tag widely used for rapid and highly specific one-step protein purification. Native elution of protein from anti-FLAG antibody resins allows the identification of protein and nucleic acid binding partners and functional analysis using biochemical activity assays.
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| Publicado no: | Methods Enzymol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4794115/ https://ncbi.nlm.nih.gov/pubmed/26096505 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2014.11.010 |
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