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Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads

FLAG is an affinity tag widely used for rapid and highly specific one-step protein purification. Native elution of protein from anti-FLAG antibody resins allows the identification of protein and nucleic acid binding partners and functional analysis using biochemical activity assays.

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Bibliografski detalji
Izdano u:Methods Enzymol
Glavni autori: Gerace, Erica, Moazed, Danesh
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4794115/
https://ncbi.nlm.nih.gov/pubmed/26096505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2014.11.010
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