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Coimmunoprecipitation of Proteins from Yeast

This protocol outlines a procedure for testing whether two proteins interact. A target protein will be immunoprecipitated using an antibody that recognizes it (or a tagged version of the protein). The immunoprecipitated material will be separated by SDS-PAGE and analyzed by Western blotting to asses...

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Bibliografische gegevens
Gepubliceerd in:Methods Enzymol
Hoofdauteurs: Gerace, Erica, Moazed, Danesh
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2014
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4370295/
https://ncbi.nlm.nih.gov/pubmed/24674059
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-420119-4.00002-1
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