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Tandem Affinity Purification and Mass Spectrometry (TAP-MS) for the Analysis of Protein Complexes

Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP) primarily based on the use of the FL...

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Библиографические подробности
Опубликовано в: :Curr Protoc Protein Sci
Главные авторы: Adelmant, Guillaume, Garg, Brijesh, Tavares, Maria, Card, Joseph D., Marto, Jarrod A.
Формат: Artigo
Язык:Inglês
Опубликовано: 2019
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC6579647/
https://ncbi.nlm.nih.gov/pubmed/30706993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpps.84
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