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Tandem Affinity Purification and Mass Spectrometry (TAP-MS) for the Analysis of Protein Complexes

Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP) primarily based on the use of the FL...

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Dettagli Bibliografici
Pubblicato in:Curr Protoc Protein Sci
Autori principali: Adelmant, Guillaume, Garg, Brijesh, Tavares, Maria, Card, Joseph D., Marto, Jarrod A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2019
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6579647/
https://ncbi.nlm.nih.gov/pubmed/30706993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpps.84
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