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Improved tandem affinity purification tag and methods for isolation of proteins and protein complexes from S. pombe

The Tandem Affinity Purification (TAP) method uses an epitope that contains two different affinity purification tags separated by a site-specific protease site to isolate a protein rapidly and easily. Proteins purified via the TAP tag are eluted under mild conditions, allowing them to be used for st...

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Podrobná bibliografie
Vydáno v:Cold Spring Harb Protoc
Hlavní autoři: Zilio, Nicola, Boddy, Michael Nicholas
Médium: Artigo
Jazyk:Inglês
Vydáno: 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5570513/
https://ncbi.nlm.nih.gov/pubmed/28250214
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot091611
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