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Engineering CRISPR-Cpf1 crRNAs and mRNAs to maximize genome editing efficiency
Cpf1, a type-V CRISPR-Cas effector endonuclease, exhibits gene-editing activity in human cells through a single RNA-guided approach. Here, we report the design and assessment of an array of 42 types of engineered Acidaminococcus sp. Cpf1 (AsCpf1) CRISPR RNAs (crRNAs) and 5 types of AsCpf1 mRNAs, and...
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| Yayımlandı: | Nat Biomed Eng |
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| Asıl Yazarlar: | , , , , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
2017
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5562407/ https://ncbi.nlm.nih.gov/pubmed/28840077 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41551-017-0066 |
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