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Design and assessment of engineered CRISPR–Cpf1 and its use for genome editing
Cpf1, a CRISPR endonuclease discovered in Prevotella and Francisella 1 bacteria, offers an alternative platform for CRISPR-based genome editing beyond the commonly used CRISPR–Cas9 system originally discovered in Streptococcus pyogenes. This protocol enables the design of engineered CRISPR–Cpf1 comp...
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| Publicado no: | Nat Protoc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6383568/ https://ncbi.nlm.nih.gov/pubmed/29622802 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2018.004 |
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