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Secondary structure in the target as a confounding factor in synthetic oligomer microarray design

BACKGROUND: Secondary structure in the target is a property not usually considered in software applications for design of optimal custom oligonucleotide probes. It is frequently assumed that eliminating self-complementarity, or screening for secondary structure in the probe, is sufficient to avoid i...

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Autores principales: Ratushna, Vladyslava G, Weller, Jennifer W, Gibas, Cynthia J
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2005
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC555549/
https://ncbi.nlm.nih.gov/pubmed/15755320
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-6-31
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